By Kewal K. Jain
Culling jointly excerpts from a variety of writings via Dr. Kewal okay. Jain on biotechnology issues as they relate to issues of the worried method, Applications of Biotechnology in Neurology covers numerous functions for these operating in lifestyles sciences and the pharmaceutical sciences, really these constructing diagnostics and therapeutics for the anxious procedure. This distinct quantity delves into components equivalent to neurobiotechnology, like neurogenomics and neuroproteomics, molecular diagnostics, quite a few equipment of bettering systemic management of gear for specified supply to the worried approach, together with using nanobiotechnology, biotechnology-based concepts and items for neuroprotection, in addition to chapters on neurosurgery and customized neurology.
Thorough, state-of-the-art, and thoughtfully geared up, Applications of Biotechnology in Neurology serves as a fantastic advisor, supplemented by way of seventy five tables and sixteen figures in addition to various references from contemporary literature in this subject, that are appended to every chapter.
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Additional resources for Applications of Biotechnology in Neurology
In de novo sequencing, short DNA fragments puriﬁed from individual bacterial colonies are individually sequenced and assembled electronically into one long, contiguous sequence. This method does not require any pre-existing information about the sequence of the DNA. Re-sequencing, using next generation technologies, means determination of variations of DNA sequence in an organism where the nominal sequence is already known. It is often performed using PCR to amplify the region of interest (pre-existing DNA sequence is required to design the PCR primers).
The technologies are described in detail in a special report on proteomics (Jain 2012). The most comprehensive source of information on proteomics is found in protein sequence databases. Currently, 2D GE patterns are scanned into a computer and then analyzed by computer algorithms that quantify the gel patterns according to whether they are obtained under normal or physiologically altered conditions. Application of Proteomics for the Study of Nervous System The term neuroproteomics is used for the application of proteomic technologies to study the nervous system and develop diagnostics as well as therapeutics for neurological disorders (Jain 2002).
Because the proteome of a cell or tissue is not a simple reﬂection of its transcriptome, direct protein-based analysis is needed. K. 1007/978-1-62703-272-8_3, © Springer Science+Business Media, LLC 2013 17 18 3 Neuroproteomics The classical approaches in proteomics involve separating proteins by two-dimensional gels and identifying visualizing bands or spots. A modiﬁcation of this technique is 2D polyacrylamide gel electrophoresis (GE). Protein can be detected by Western blot or ﬂuorescence detection methods.