Applications of Ion Chromatography in the Analysis of by Lokesh Bhattacharyya, Jeffrey S. Rohrer

By Lokesh Bhattacharyya, Jeffrey S. Rohrer

It is a accomplished resource of knowledge at the program of ion chromatography (IC) within the research of pharmaceutical medicinal drugs and biologicals. This publication, with individuals from academia, pharma, the biotech undefined, and tool production, offers different views, adventure, and services of the idea leaders of IC in a entire demeanour. It explores capability IC purposes in several points of product improvement and qc checking out. additionally, an appendix part provides info on severe actual and chromatographic parameters on the topic of IC and knowledge on present brands of IC platforms, columns, and different elements.

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Conceptually, there are two possibilities: direct and indirect detection. Direct detection involves the measurement of the absorption of the analyte, normally against a background of low absorbance by the eluent. On the other hand, indirect detection involves monitoring the background absorbance of the eluent under conditions where this absorbance will be modified (normally by diminution) by the presence of an analyte. Direct detection is therefore analyte-specific in that only analytes which absorb light at the detection wavelength will be detected, whereas indirect detection is usually general in nature in that all analytes will be detected.

An organic acid having strong affinity towards microporous, fully sulfonated PS-DVB stationary phases is benzoic acid, which shows very strong retention in comparison to aliphatic mono- and di-carboxylic acids having similar pKa values. 4b where the retention time for benzoic acid in 2 mM H2 SO4 is about eight times higher than acetic acid, although the latter is the weaker acid. Under the current mechanism for IEC, this increased retention would be attributed to adsorption onto the unfunctionalized portions of the polymer support phase.

J. Chromatogr. A 1997;782:33–40. 24. Lee DP, Lord AD. A high performance phase for the organic acids. LC-GC 1987;5: 261–266. 25. Papadoyannis IN, Samanidou VF. Encyclopedia of Chromatography, Marcel, New York: Dekker; 2001. p 455–457. 26. Tanaka K, Haddad PR. Ion exclusion chromatography: liquid chromatography. In: Wilson ID, Adlard ER, Cooke M, Poole CF, editors. Encyclopedia of Separation Science, San Diego: Academic Press; 2000. p 3193–3201. 27. Ng KL, Paul B, Haddad PR, Tanaka K. Retention modeling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography.

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