Applied molecular biotechnology : the next generation of by Muhammad Sarwar Khan, Iqrar Ahmad Khan, Debmalya Barh

By Muhammad Sarwar Khan, Iqrar Ahmad Khan, Debmalya Barh

Applied Molecular Biotechnology: the following new release of Genetic Engineering explains cutting-edge advances within the swiftly constructing zone of molecular biotechnology, the know-how of the recent millennium. made from chapters authored through prime specialists of their respective fields, this authoritative reference text:

  • Highlights the newest omics-based instruments and ways utilized in sleek biotechnology
  • Explains how a number of molecular biology applied sciences can be utilized to increase transgenic vegetation and the way these crops can meet growing to be nutrients and plant-derived product demands
  • Discusses chloroplast gene expression platforms, mitochondrial omics, plant practical genomics, and whole-genome resequencing for crop improvement
  • Explores plant–microbe and plant–insect interactions affecting plant defense and productivity
  • Covers animal types, pharmacogenomics, human tissue banking, and the molecular prognosis of illnesses equivalent to cervical melanoma, weight problems, and diabetes
  • Examines the molecular points of viral illnesses, construction of commercial commodities utilizing viral biotechnology, and biotechnological makes use of of magnetic nanoparticles
  • Describes using biotechnology within the nutrition, chemical, pharmaceutical, environmental conservation, and renewable power sectors

Applied Molecular Biotechnology: the subsequent iteration of Genetic Engineering serves as a springboard for brand new discoveries in molecular biology and its functions. hence, this booklet is a useful source for college kids and researchers of molecular biotechnology.

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Genetic transformation mediated by this bacterium is a major tool for obtaining many commercially important transgenic crops. The Ti plasmid harbored by wild-type A. tumefaciens strains range between 200 and 800 kb in size. The transfer DNA or T-DNA and virulence (vir) genes regions are essential features of the Ti plasmids and facilitate tumor induction in plant tissue. The tumors or 36 Applied molecular biotechnology crown gall disease is caused by the expression of genes coding for phytohormones such as auxins and cytokines present in the T-DNA.

3. Size exclusion chromatography: Separates proteins according to size. The stationary solid phase consists of beads containing pores of different dimensions. Small proteins enter the pore of beads and migrate through the column slower than the particles of larger size and consequently emerge in the later fractions. 4. Absorption chromatography: Proteins to be separated are loaded on the column and sequentially released and eluted by disrupting the interaction between protein and stationary phase.

MALDI has emerged as an efficient and versatile technique for the analysis of biomolecules and large organic molecules. Chromatographic techniques Chromatography is an important method for separating mixture of compounds and isolate different components. It follows the principle of different rate of mobility and resistance faced by the migration and selective retardation of the solute during passage through resin particles. It comprises two phases, that is, mobile and stationary phase. Diverse chromatographic methods are present, which allow purification of different biomolecules.

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